DE2 (cyclone2 EP2C35F672C6) can compile successful but cant run on board

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Actually I did pin assignment by import the DE2_pin_assignments.csv.

It did work when I applied on Quartus 10.1

Actually I did try to assign the pins manually with pin planner. And, it did work.

I wonder whether the .csv file I used is not compatible with the Quartus 12.1

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You should be able to check with the Pin Planner whether the assignments for those two signals are correct.

Cheers,

Dave