Hello, I've replaced cyclone 3 fpga with cyclone 5. But I'm confused pin assignments I made. Q1) Is there any way to verify pin assignments I've done? Q2) Can quartus prime used for verifying pin assignments? Thanks in Advance!

In that case, you can follow here:

https://www.intel.com/content/www/us/en/homepage.html -> product -> FPGA and Programmable device -> cyclone V(example) -> Documentations and Support ->

Cyclone V Device Family Pin Connection Guidelines (PDF)

Device Pin-Outs

Do this for the same for cyclone III for comparison. After review the above, iF Quartus able to compile without any timing error and Fitter error. it should be good to go.

Thanks