the system can not find the PCI Board??

hello, girls and guys. During these days, i have designed some small examples with Altera PCI board.(EP2C35).One of my designs contains the following parts:

a PCI IP(Master/Slave,32 bit, 66MHz);

a DMA Controller;

a DDR2 SDRAM;

a PLL( generate the requried clock for the DDR2 Controller).

i have completed the compilation with QuartusII and Sopc Builder. Because of the lisence problem, i can only generate the time limited sof file. so i downloaded my design through the Jtag connector to the FPGA chip. however the system can not find the board, and it can not give some information like "find new hardware".

i don't know why. so after plugging the board into the PCI slot, download the .sof file into the FPGA, restart my computer(not shut down), but the system still can not see the board.

while for the factory design, which stored into the EPCS64, when power on, the system will give some tips like" find new hardware", and it will direct you to find the proper driver.

i need to check my design again, but i really want to listen your ideas and advice.

Expecting your inspration and experience!

thanks in advance!