Quartus assumes the single ended signal is going to cause interference on the differential signal because it's too close.
Add an '
io_maximum_toggle_rate "0mhz"' attribute to any non-differential signals in that bank. Do this as follows:
In the 'Pin Planner', find the relevant signal - 'TX2_SDA'. In the 'Toggle Rate' column, enter 0 (zero). Do this for any non-differential signal in the same bank as 'tof_n'. If you can't see the 'Toggle Rate' column, right click anywhere in the column headings and select 'Customise Columns...', find 'Toggle Rate' in the 'Available columns:' box and add it to the visible columns.
The JTAG signals (TMS, TDO etc.) are reserved pins - you don't need to assign them.
I don't understand what you mean by the 'ek' signal.
Cheers,
Alex