how to generate signal in matlab and implement in hardwave

The question isn't particularly clear.

A signal processing application will have input and output signals, e.g. input from an ADC and output to a DAC. You intend to replace the ADC input by a generated test signal? How about the output, where does it go?

You can store the test signal in internal RAM, either compile it into the design or load it at runtime through a digital interface.