NIOS 2 on Altera's Stratix II GX PCI-E dev board not working

I am currently using:

Quartus II 7.1 full version with full license.

Open Core plus.

and I am using Altera's Stratix II GX PCI-E dev board to dev nios2 system.

my NIOS 2 system is not functioning. It only includes nios2, JTAG UART, PIO, onchip ram and PLL to slow down the clock.

I used the hellow pio example in the nios IDE. not working.

hellow world small example, not working.

when I check run the hellow world application in Hardware, I got the following error from the IDE console:

Using cable "USB-Blaster [USB-0]", device 2, instance 0x00

Pausing target processor: not responding.

Resetting and trying again: FAILED

Leaving target processor paused

do you have this problem you are using this board for nios 2 development?

The PLL output works. I attached my customer LED IP to the system that only get the clk and I can see blinking.

I also simulated the entire system in ModelSIM and it works since the hello world is printed to the JTAG console and I can see the waveform from the ModelSIM. my PIO LED is working.

I have no idea how to fix this problem.

Thank you.