Hi
Thanks for update. 1) Windows Server 2016 version is not an officially support OS for Questa. An unsupported platform means that some things may work, but others not, and we won't put any resources into debugging or diagnosing. We recommended to use the right OS for Questa.
https://www.intel.com/content/www/us/en/support/programmable/support-resources/design-software/os-support.html
2) And could you help re-confirm your environment variable setup again? You need to set your LM_LICENSE_FILE environment variable to point to the license that Intel provides. c:\intel\license.dat or something similar.
You can refer to link on how to set environment variable.
To make this entry, you may perform a windows Search for "System Variables" and then click on "Edit the System Environment Variables" and then click "Environment Variables". Be aware that you must add the entry in System variables, not user variables.
https://www.intel.com/content/www/us/en/docs/programmable/683472/21-4/environment-variables.html
Specifying the License for the Questa
https://www.intel.com/content/www/us/en/docs/programmable/683472/21-4/specifying-the-license-for-the-software.html
Finally, you must allow firewall access to those port numbers.